Source
Espeland M, Irestedt M, Johanson KA, Akerlund M, Bergh JE, Kallersjo M. (2010) Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections. Frontiers in Zoology 7:2.
Abstract
Background: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, paradichlorobenzene and naphthalene on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time intervals.
Results: The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not.
Conclusion: Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should ,however, be preferred due to physical damage of specimens and putative health risks by chemicals.
Results: The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not.
Conclusion: Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should ,however, be preferred due to physical damage of specimens and putative health risks by chemicals.
簡介
在昆蟲標本的典藏上，二氯松(Dichlorvos)、對二氯苯(paradichlorobenzene)、萘(naphthalene)為常見的除蟲劑主要成份，但此三種化學物質對於標本DNA之影響卻沒有被討論過。此篇文章以家蠅(Musca domestica)為對象，測試標本在三種不同防蟲劑及不同濃度下其DNA萃取及增幅的表現。結果顯示暴露在二氯松之標本，不管是DNA的萃取或是粒線體DNA及核DNA的增幅均產生負面的影響，且影響隨著時間的增長而加劇；而暴露在二氯苯及萘之標本在此實驗中(為期20個月)並無顯著影響。因此認為含有二氯松之除蟲劑不適合用在昆蟲標本之典藏。
